Natalia Vorobyeva
Far Eastern Federal University, Russia
Title: Effect of phospholipids from echinoderms in content of TI-complexes on immunogenicity of human serum albumin
Submitted Date: 9/15/2015
Biography
Natalia Vorobyeva graduated from Far Eastern Federal University (FEFU), Department of Biochemistry, Microbiology and Biotechnology of FEFU (specialty Biochemistry) at 2011. After postgraduate training at the same Department of FEFU (Supervisor Prof. Sanina N.M.), she defended her PhD thesis “Influence of polar lipids and triterpene glycosides from marine organisms on the conformation and immunogenicity of protein antigens of tubular immunostimulatory complexes†in 2015 at Pacific Institute of Bioorganic Chemistry, Vladivostok. She has 33 publications including 5 articles in refereed journals and 1 patent
Abstract
We previously demonstrated the possibility of using tubular immunostimulating complex (TI complex) based on monogalactosyldiacylglycerol (MGDG) from marine macrophytes, cholesterol and cucumarioside A2-2 of the holothurian Cucumaria japonica as an adjuvant carrier of membrane protein antigens. Present work aims to test the possibility of using TI-complexes as an adjuvant of hydrophilic antigens, which will greatly, simplifies the production of vaccines based on TI-complexes and extends the range of their application. Human serum albumin (HSA) was used as a model soluble antigen. For preparing of TI-complexes we used glycolipid MGDG from the seaweed Ulva lactuca and phospholipids phosphatidylcholine (PC) and phosphatidylethanolamine (PE) from marine invertebrates starfish Distolasterias nippon and sea urchin Strongylocentrotus intermedius. Substitution of MGDG for PC from D. nippon, enriched by n-3 PUFAs, did not change the immunostimulatory activity of TI-complex at immunization of animals. However, the use of other phospholipids with predominance of n-6 PUFAs for modification TI-complex resulted in the reduction of the adjuvant activity of complex as compared with one, containing PC from D. nippon, and HSA alone. TI-complex based on PE from S. intermedius had the strongest suppressive effect. The greatest changes in the content of cytokines IL-6, IL-12 and GM-CSF depending on lipid component of the TI-complexes indicate the possibility to regulate the T-cell immune response