Techniques Involved in Lipid Research

There are varied methodologies as biological tissues are not alike when considering their structure, texture, sensitivities and lipid contents. The ideology behind the solvent extraction is to completely extract all the lipid components from a sample, leaving the other components behind which always depends on the polarity of the lipids present. 

These techniques like classical Bligh & Dyer lipid extraction, chemical extractions by means of diverse solvents and sonication, direct saponification and supercritical CO2 extraction used to extract cellular or liquefied lipids from the other components like proteins, polysaccharides, small molecules (amino acids, sugars...) and use them for advanced investigations.

  • Polar lipids
  • Amino acids
  • Polysaccharides
  • Proteins
  • Gas Chromatography
  • High Pressure Liquid Chromatography (HPLC)
  • Thin-Layer Chromatography (TLC)
  • Immunoassays
  • Electrophoresis (SDS-PAGE, IEF, non-denaturing electrophoresis)
  • Chromatography

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